Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 3;9:598. doi: 10.3389/fpls.2018.00598

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Shivalingamurthy, Anangi, Kalaipandian, Glassop, King and Rae.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Recombinant ShINH1 expression in E. coli. (A) Schematic of construct encoding a His₆-MBP: ShINH1 fusion protein for production of recombinant ShINH1 in E. coli. SDS-PAGE gels showing expression and purification of recombinant ShINH1: (B) Production and NiNTA purification of recombinant ShINH1 and (C) TEV cleavage and purification of recombinant ShINH1 through gel filtration column chromatography. Lane1: Pre induction sample; Lane2: IPTG induction sample; Lane3: Insoluble fraction; Lane4: Soluble fraction; Lane5: flow through; Lane6: 10 mM imidazole wash; Lane7: 30 mM Imidazole wash; Lane8: 300 mM imidazole wash; Lane9: Molecular size marker (Novex Sharp pre-stained protein ladder; Thermofisher Scientific, USA); Lane10: TEV protease treated sample; Lane12: Fraction containing cleaved His₆-MBP and ShINH1; Lane13: Molecular size marker; Lanes14–24: Purified ShINH1 Samples.