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. 2018 May 3;9:598. doi: 10.3389/fpls.2018.00598

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Copyright © 2018 Shivalingamurthy, Anangi, Kalaipandian, Glassop, King and Rae.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purification and characterization of recombinant ShINH1. (A) Fractionation of recombinant ShINH1 using gel filtration chromatography. A HiTrap S-200 prepacked gel filtration column was used to separate TEV cleaved recombinant ShINH1 (peak 3) from uncleaved (His₆-MBP: ShINH1 as peak 1) and His₆-MBP (peak 2) fractions shown in the chromatogram. (B) Circular dichroism (CD) spectrum of recombinant ShINH1 obtained at 25°C.The CD spectrum contains minima at 222 and 209 nm, which are diagnostic of α-helical secondary structure.