Table 1.
Sequences of primers used for gene isolation, transcript expression assessment, vector construction for GFP localization, and recombinant protein expression.
| Primer # | Orientation | Use | Sequence (5′-3′) |
|---|---|---|---|
| 1 | Forward | INH1 gene isolation | ATGAAGCTTCTGCAAGCTCTG |
| 2 | Reverse | INH1 gene isolation | CTACAGCGCCTCCGTTACAGA |
| 3 | Forward | INH2 gene isolation | ATGAAGCTAGTCTGCTCCGTG |
| 4 | Reverse | INH2 gene isolation | TTAATCACTAATCTTTGGCCT |
| 5 | Forward | INH1 qRTPCR | CGTCCAACGCTTCCGTCTTA |
| 6 | Reverse | INH1 qRTPCR | GTCGGCCTGGAAGAACTTGA |
| 7 | Forward | ADF qRTPCR | CTACTACTGTGGATTTGTACGCCATTATAG |
| 8 | Reverse | ADF qRTPCR | GGACCTTTTTTACACAGCAACAAAC |
| 9 | Forward | INH1-GFP vector construct preparation | TATAACTAGTATGAAGCTTCTGCAAGCTCTG |
| 10 | Reverse | INH1-GFP vector construct preparation | GCGGCCGCTTACTACAGCGCCTCCGTTACAGA |
| 11 | Forward | PLIC INH1 expression vector construct preparation | TATATGGTACCGAGAACCTGTACTTCCAATC |
| 12 | Reverse | PLIC INH1 expression vector construct preparation | ACATAGAGCTCTTACTACAGCGCCTCCGTTAC |