Figure 3.

Effect of pyruvate concentration in the mitochondrial isolation media and subsequent treatment of isolated leaf mitochondria with diamide on the ratio of the levels of reduced and oxidized forms of the AOX protein present. Diamide treatment of mitochondria and quantification of AOX protein levels were described in “Materials and Methods.” The data are the average from 3 (0 mm pyruvate), 1 (0.5 mm pyruvate), 2 (2 mm pyruvate), and 1 (5 mm pyruvate) separate mitochondrial isolations. White bars, No diamide; shaded bars, plus diamide.