Figure 5.

The effect of pyruvate on the ratio of the reduced to oxidized forms of the AOX protein present in transgenic tobacco (B9) leaf mitochondria. Mitochondria were isolated with 5 mm pyruvate present in all of the isolation media and subsequently washed in media either also containing 5 mm pyruvate or lacking pyruvate. Immediately following the wash (time 0) and 1 h (on ice) after the wash (time 1 h), the ratio of the level of reduced to oxidized form of AOX protein present was determined. These data are from the same mitochondria analyzed in Figure 4. Representative results are shown. White bars, (+)-Pyruvate washed; shaded bars, (−)-pyruvate washed.