Figure 8.

Immunoblot of the AOX protein present in a cellular protein extract from transgenic (B9) tobacco suspension cells d 3 after subculture. Protein extracts were obtained from either untreated control cells (C), cells treated for 15 min with 1 μm FCCP, or cells treated for 10 min with 20 mm H₂O₂, and 40 μL of extract was subsequently analyzed by non-reducing SDS-PAGE and immunoblot analysis as described in “Materials and Methods.” The lane marked “M” is a sample of protein from isolated mitochondria to clearly show the position of the oxidized and reduced forms of the AOX protein. The arrows at left indicate the position of the oxidized (upper arrow) and reduced (lower arrow) forms of the AOX protein. Representative results are shown.