Table I.

O₂ uptake by transgenic tobacco (B9) leaf mitochondria isolated in the absence of pyruvate

Run	Sequential Additions	O2 Uptake
		nmol O2 mg−1 protein min−1
1	NADH	49 ± 2
	ADP	139 ± 8
	Myxothiazol	5 ± 2
	Pyruvate	10 ± 1
	DTT	49 ± 3
	n-Propyl gallate	0 ± 0
2	Pyruvate	NDa
	NADH	57 ± 4
	ADP	143 ± 10
	Myxothiazol	14 ± 1
	DTT	52 ± 7
	n-Propyl gallate	0 ± 0

Rates of O₂ uptake were determined in an O₂ electrode cuvette after sequential addition of the compounds indicated. Note that in run no. 1, pyruvate was added after several minutes of NADH oxidation, while in run no. 2, pyruvate was added to the mitochondria 1 min prior to the addition of NADH. Compounds were added at the following final concentrations: 2 mm NADH, 1 mm ADP, 16 μm myxothiazol, 2 mm pyruvate, 10 mm DTT, and 100 μm n-propyl gallate. Data are the average ± sd (n = 3) using mitochondria from the same isolation. Separate mitochondrial isolations yielded similar results.

^aND, Not determined.