Fig. 4.

RINCK mediates the monoubiquitination of cGAS. a HEK293T cells were transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-Flag antibody and then immunoblotted with indicated antibodies. b, c HEK293T cells were transfected with indicated plasmids for 24 h. Cell lysates were immunoblotted with indicated antibodies. d HEK293T cells were transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-HA antibody and then immunoblotted with indicated antibodies. e HEK293T cells were transfected with indicated plasmids for 24 h. Cell lysates were immunoblotted with the indicated antibodies. f HEK293T cells were transfected with indicated plasmids for 24 h. Cell extract was collected for quantification of cGAMP by LC–MS/MRM. Data are presented as the mean ± SD. ***P < 0.001. N.D., not detected. Data represent three independent experiments