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. 2018 Apr 26;2018:7569127. doi: 10.1155/2018/7569127

Figure 1.

Figure 1

L-Arginine enhances protein synthesis by phosphorylating mTOR (Thr 2446) and p70S6K (Thr 389) in C2C12 cells. The protein synthesis rate was evaluated after treatment by supplementation with puromycin (10 μM) for 30 min in the cell-free supernatant (a). The levels of phosphorylated mTOR (b) and p70S6K (c) in the C2C12 cells cultured for 36 h in the presence of 1 mM of L-arginine. When the total protein bands showed significant differences with different treatments, the phosphorylated protein bands were normalized to the total protein bands. In contrast, if the total protein bands were similar across different groups, both the phosphorylated and total protein bands were normalized to β-actin. Data are presented as the means ± SEM (n = 6); ∗∗P < 0.01 and ∗P < 0.05 compared with untreated cells. NS, P > 0.05.