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. 2018 Apr 26;2018:7569127. doi: 10.1155/2018/7569127

Figure 2.

Figure 2

L-Arginine supplementation increases and L-NAME supplementation decreases NO concentrations in C2C12 cells and the culture medium, as well as the activities of inducible nitric oxide synthase (iNOS) and total nitric oxide synthase (TNOS) in C2C12 cells (U/mg protein). NO abundance in C2C12 cells (a) and culture medium (b) was detected after 3 h, 18 h, and 36 h treatments with L-arginine or L-NAME. The activity of NOS-iNOS (c) and TNOS (d) was analyzed following L-arginine or L-NAME supplementation for 3 h, 18 h, and 36 h in C2C12 cells. Data are expressed as the means ± SEM (n = 7). ∗∗P < 0.01 compared with untreated cells. NS, P > 0.05.