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. 2018 Apr 26;2018:7569127. doi: 10.1155/2018/7569127

Figure 6.

Figure 6

L-NAME and SNP, respectively, inhibit and activate protein synthesis and the phosphorylation of mTOR (Thr 2446) and p70S6K (Thr 389) in C2C12 cells. The protein synthesis rate was evaluated following treatment by supplementation with puromycin (10 μM) for 30 min in the cell-free supernatant (a). The phosphorylation levels of mTOR (b) and p70S6K (c) in C2C12 cells cultured for 36 h in the presence of 1 μM of SNP and 10 mM of L-NAME. When the total protein bands showed significant differences with different treatments, the phosphorylated protein bands were normalized to the total protein bands. In contrast, if the total protein bands were similar across different groups, both the phosphorylated and total protein bands were normalized to β-actin. Data are presented as the means ± SEM (n = 6). ∗∗P < 0.01 and ∗P < 0.05 compared with untreated cells. NS, P > 0.05.