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. 2018 Apr 12;9(18):4258–4267. doi: 10.1039/c8sc00832a

Fig. 1. (A) Agarose gel electrophoresis analysis of the HRCA products. Lane M is the DNA ladder marker. Lanes 1–3 represent HRCA products in the presence of circular template-21 (lane 1), circular template-21 + miR-21 (lane 2) and circular template-21 + miR-221 (lane 3). Lanes 4–6 represent the HRCA products in the presence of circular template-221 (lane 4), circular template-221 + miR-221 (lane 5) and circular template-221 + miR-21 (lane 6). Lanes 7 and 8 represent the HRCA products in the presence of circular template-21 + circular template-221 (lane 7) and circular template-21 + miR-21 + circular template-221 + miR-221 (lane 8). (B) Fluorescence measurement of the HRCA products with SYBR Gold as the fluorescent indicator in the presence of circular template-21 (red line), circular template-21 + miR-21 (blue line) and circular template-21 + miR-221 (black line). (C) Fluorescence measurement of the HRCA products with SYBR Gold as the fluorescent indicator in the presence of circular template-221 (red line), circular template-221 + miR-221 (blue line) and circular template-221 + miR-21 (black line). (D) Fluorescence measurement of the HRCA products with SYBR Gold as the fluorescent indicator in the presence of circular template-21 + circular template-221 (red line) and circular template-21 + miR-21 + circular template-221 + miR-221 (blue line). The miR-21 concentration is 0.2 nM, the miR-221 concentration is 0.2 nM, the circular template-21 concentration is 10 nM and the circular template-221 concentration is 10 nM.

Fig. 1