Table 1. Sequences of the oligonucleotides a .
Note | Sequence (5′–3′) |
miR-21 | UAG CUU AUC AGA CUG AUG UUG A |
miR-221 | AGC UAC AUU GUC UGC UGG GUU UC |
Circular template-21 | CAG AAC AGC ACA AGA CAG GAC AAG ACA CAC GCC GAA TCA ACA TCA GTC TGA TAA GCT ACC AGA CAG ACG A |
Circular template-221 | CAG AAC AGC ACA AGA CAG GAC AAG ACA CAC GCC GAA GAA ACC CAG CAG ACA ATG TAG CTC CAG ACA GAC GA |
Reverse primer | GAC AGA CGA CAG AAC AG |
Capture probe | Biotin – GGC GTG TGT CTT GTC CTG |
Reporter probe-21 | TAG CTT ATC AGA CTG ATG TTG A – Cy3 |
Reporter probe-221 | AGC TAC ATT GTC TGC TGG GTT TC – Texas Red |
Let-7a | UGA GGU AGU AGG UUG UAU AGU U |
RNA-1 | UAG CUU AUC ACA CUG AUG UUG A |
RNA-2 | AGC UAC AUU GUC GGC UGG GUU UC |
aIn the circular template, the hybridization region for target miRNA is shown in bold. The underlined characters of RNA-1 represent the different base compared with target miR-21. Underlined characters of RNA-2 represent the different base compared with target miR-221.