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. 2018 Apr 12;9(18):4258–4267. doi: 10.1039/c8sc00832a

Table 1. Sequences of the oligonucleotides a .

Note Sequence (5′–3′)
miR-21 UAG CUU AUC AGA CUG AUG UUG A
miR-221 AGC UAC AUU GUC UGC UGG GUU UC
Circular template-21 CAG AAC AGC ACA AGA CAG GAC AAG ACA CAC GCC GAA TCA ACA TCA GTC TGA TAA GCT ACC AGA CAG ACG A
Circular template-221 CAG AAC AGC ACA AGA CAG GAC AAG ACA CAC GCC GAA GAA ACC CAG CAG ACA ATG TAG CTC CAG ACA GAC GA
Reverse primer GAC AGA CGA CAG AAC AG
Capture probe Biotin – GGC GTG TGT CTT GTC CTG
Reporter probe-21 TAG CTT ATC AGA CTG ATG TTG A – Cy3
Reporter probe-221 AGC TAC ATT GTC TGC TGG GTT TC – Texas Red
Let-7a UGA GGU AGU AGG UUG UAU AGU U
RNA-1 UAG CUU AUC ACA CUG AUG UUG A
RNA-2 AGC UAC AUU GUC GGC UGG GUU UC

aIn the circular template, the hybridization region for target miRNA is shown in bold. The underlined characters of RNA-1 represent the different base compared with target miR-21. Underlined characters of RNA-2 represent the different base compared with target miR-221.