Figure 5. Effects of M and Z on Pam3CSK4-induced IL-6 formation in RAW 264.7 cells. Cells (5×104/cm2) were treated with M, Z, MZ, or IBU for 30 minutes before treatment with 10 μg/mL Pam3CSK4 for 24 hours at 37°C. The data are expressed as mean±standard deviation, as obtained from 6 separate experiments.
M: soft 75% ethanol Magnoliae Cortex extract (60 μg/mL in 1% DMSO), Z: titrated unsaponifiable maize extract fraction (300 μg/mL in 1% DMSO), Pam3CSK4: synthetic Toll-like receptors of Porphyromonas gingivalis, IL-6: interleukin-6, MZ: the combination treatment of M and Z, IBU: ibuprofen (10 mM in 1% DMSO), DMSO: dimethyl sulfoxide.
a)P<0.05 in comparison with Pam3CSK4; b)P<0.05 in comparison with the control; c)P<0.05 in comparison with M.