Figure 8. Effects of M and Z on Pam3CSK4-induced NF-κB transactivation in RAW 264.7 cells. Cells (5×10⁴/cm²) were treated with M, Z, MZ, or IBU for 30 minutes prior to treatment with 10 μg/mL Pam3CSK4 for 2 hours at 37°C. The data are expressed as mean±standard deviation, as obtained from 6 separate experiments.

M: soft 75% ethanol Magnoliae Cortex extract (60 μg/mL in 1% DMSO), Z: titrated unsaponifiable maize extract fraction (300 μg/mL in 1% DMSO), Pam3CSK4: synthetic Toll-like receptors of Porphyromonas gingivalis, NF-κB: nuclear transcription factor κB, MZ: the combination treatment of M and Z, IBU: ibuprofen (10 mM in 1% DMSO), DMSO: dimethyl sulfoxide, p65: transcription factor p65.

^a)P<0.05 in comparison with Pam3CSK4; ^b)P<0.05 in comparison with the control; ^c)P<0.05 in comparison with M and Z.