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GLUT4 translocation to the plasma membrane. For the evaluation of the GLUT4 L6-GLUT4myc, cells (150,000 cell/well) were exposed to MeOH extract for 20 h. Serum depleted cells were treated without (−) or with (+) 1 μM insulin for 20 min at 37°C and surface myc-tagged GLUT4 density was quantified using the antibody coupled colorimetric assay. Values given represent means ± SEM (relative to untreated control cells) of three independent experiments carried out in triplicate.
