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. 2018 May 3;9:955. doi: 10.3389/fimmu.2018.00955

Figure 3.

Figure 3

Distribution and tracking of the T-cell receptor β (TCRβ) immune repertoire in peripheral blood mononuclear cells (PBMC) and subcutaneous metastasis from patient #006. (A) Percentage of total TCRβ clones covered by the TOP100 clones, the 100 most frequent ones (nucleotide sequences). (B) Total TCRβ clones (%), from highest to lowest, in function of their cumulative frequency (nucleotide sequences). (C) The proportion of TCRβ clones (%) that constitute the TOP-25 or TQ clones, and the TOP-50 clones are shown (TOP-25/50, the proportion of clones that make up the top cumulative frequency of 25/50). (D) Pattern distribution of TCRβ clones in total PBMC, total tumor-infiltrating lymphocytes (TIL), and Top-Quartile TIL (TQ-TIL); the number of clones for each pattern and total clones analyzed in each case is indicated. Patterns: 1, present at baseline, increases in time; 2, absent at baseline, increases in time; 3, absent at baseline, detected at POST-3 sample; 4, present at baseline, decreases in time; 5, present at baseline, absent in time; and 6, absent at blood, present in tumor. (E) Common sequences among PBMC-PRE, PBMC-P1, PBMC-P3, and TIL samples. (F) The proportion of TIL clones present in blood at different times. (G) Venn diagram illustrating common clones among TIL and POST-4 samples regarding amino acid sequences. (H) Distribution in tracking patterns of total and TQ-TIL clones shared with PBMC POST-4. Pattern 3: absent at baseline, detected at POST-4 sample.