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Complementation of snf1 mutant cells by various WPK4 constructs. Cells of S. cerevisiae MCY1846 (snf1) harboring pWPK4, pASWPK4, pWPKM4, WPK4ΔC, or pSUC2 were streaked on agar plates supplemented with 2% (w/v) Suc. Cells of MCY1846 were grown at 30°C for 6 d.
