Figure 6.

Antagonistic effects of cytokinins and Suc. Total RNAs were isolated from the indicated samples and subjected to RNA-blot hybridization. A, Seven-day-old seedlings grown in water were treated with 100 μm BA for the indicated time periods. B, Seven-day-old seedlings grown in water were transferred to water containing 0.7% (w/v) Suc and harvested after the indicated time periods. C, Seven-day-old seedlings grown in water were transferred to water containing 0.7% (w/v) Suc and 100 μm BA for the indicated time periods. The hybridization probes were a 0.8-kb WPK4-specific sequence and a 1.2-kb wheat actin cDNA. Relative mRNA levels were densitometrically estimated for samples extracted from seedlings grown in water treated with BA (●) or Suc (▵) or both (○).