Table 1.
A summary of properties from different hepatocyte sources: animal, human cell line, primary human, and iPSC-derived.
| Hepatocyte Source |
Animal | HepG2 | HepaRG | Primary Human |
Cryopreserved Human |
iPSC- derived |
|---|---|---|---|---|---|---|
| Viability | Medium to High* | High | High | Medium to High* | Medium | High |
| Attachment to ECM | High | High | High | High | Medium | High |
| CYP450 Activity | Medium | Low | Medium | High | High | Low to Medium |
| Longevity in Culture | Medium | High | High | Medium | Low to Medium | Low |
| 1OATP/NTCP | High | Low | Medium | High | Medium | Low to Medium |
| Cost | Medium | Low | Low | High | High | High |
| Availability | Medium | High | High | Low | Medium | Medium |
| References | 45–47, 49 | 52, 53, 55 | 53, 55, 56, 58–60 | 23–25 | 35, 36, 39, 41 | 69–71 |
*
Dependent on efficiency and time of isolation procedure.
1
OATP (organic anion transporting polypeptide), NTCP (sodium taurocholate co-transporting polypeptide).