Table 2.
Summary of thermal denaturation data.a
| Variant | TM (°C) | ΔTM (°C) | ΔTM(O) (°C) |
|---|---|---|---|
| WW 1 | 57.6 (± 0.6) | ||
| 1(R17β3R) | 53.4 (± 0.9) | −4.2 (± 1.1) | |
| 1(S18β3S) | 59.1 (± 0.5) | 1.5 (± 0.8) | |
| 1(S19β3S) | 44.4 (± 0.2) | −13.2 (± 0.6) | |
| 1(G20βG) | 54.7 (± 0.2) | −2.9 (± 0.6) | |
| 1(R17[S,S]ACPC) | 54.3 (± 0.3) | −3.3 (± 0.7) | 0.9 (± 0.9) |
| 1(S18[S,S]ACPC) | 59.3 (± 0.0) | 1.7 (± 0.6) | 0.2 (± 0.5) |
| 1(S19[S,S]ACPC) | 43.4 (± 0.6) | −14.2 (± 0.9) | −1.0 (± 0.6) |
| 1(G20[R,R]ACPC) | 68.5 (± 0.2) | 10.9 (± 0.6) | 13.8 (± 0.2) |
| 1(G20[S,S]ACPC) | 37.0 (± 0.4) | −20.6 (± 0.7) | −17.7 (± 0.4) |
| WW 2 | 77.5 (± 0.3) | ||
| 2(A17β3A) | 68.4 (± 0.2) | −9.1 (± 0.3) | |
| 2(D18β3D) | 58.2 (± 0.5) | −19.3 (± 0.6) | |
| 2(G19βG) | 60.2 (± 0.1) | −17.3 (± 0.3) | |
| 2(A17[S,S]ACPC) | 77.6 (± 0.3) | 0.1 (± 0.4) | 9.2 (± 0.3) |
| 2(D18[S,S]ACPC) | 58.9 (± 0.4) | −18.6 (± 0.5) | 0.7 (± 0.7) |
| 2(G19[R,R]ACPC) | 74.5 (± 0.2) | −3.0 (± 0.3) | 14.2 (± 0.2) |
| WW 3 | 67.4 (± 0.4) | ||
| 3(N17β3N) | 48.5 (± 0.3) | −18.9 (± 0.5) | |
| 3(G18βG) | 48.4 (± 0.4) | −19.0 (± 0.5) | |
| 3(N17[R,R]ACPC) | 77.0 (± 0.4) | 9.7 (± 0.5) | 28.5 (± 0.5) |
| 3(G18[R,R]ACPC) | 59.1 (± 0.2) | −8.2 (± 0.4) | 10.7 (± 0.4) |
a
Thermal denaturation data for all WW domain variants reported in this study. TM values are mean values of three replicates, with standard deviations shown in parentheses. For each substituted variant, ΔTM represents difference in TM relative to corresponding parent sequence. For ACPC-containing variants, ΔTM(O) represents difference in TM relative to variant containing β3 residue.