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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Biomaterials. 2018 Apr 18;171:207–218. doi: 10.1016/j.biomaterials.2018.04.031

Fig. 2.

Fig. 2

Non-viral vectors for in vitro CRISPR/Cas9 delivery. (A) Rational design of arginine nanoparticles (ArgNPs) for intracellular delivery of engineering Cas9 protein (Cas9En) or Cas9En/sgRNA ribonucleoprotein complexes (RNPs) via membrane fusion. Engineering Cas9 protein was constructed to carry an N-terminus E-tag and a C-terminus nuclear localization signal (NLS). Reprinted with permission from ref. 97. Copyright 2017 American Chemical Society. (B) The schematic process of preparation, cellular uptake, endosomal eacape and genome editing of zeolitic imidazole frameworks-based CRISPR/Cas9 (CC-ZIFs) delivery. Reprinted with permission from ref. 98. Copyright 2017 American Chemical Society. (C) Design of modular RNA aptamer-streptavidin complexes (S1mplexes) for co-delivery of Cas9/sgRNA ribonucleoprotein complexes and ssODN donor template. Reprinted with permission from ref. 99. Copyright 2015 Nature Publishing Group. (D) The schematic process of preparation and intracellular delivery of GO-PEG-PEI based Cas9/sgRNA delivery. The confocal laser scanning images and the quantification of fluorescence intensity indicated down-regulated expression of EGFP protein after targeted knockout of EGFP genes in AGS.EGFP cells with GO-PEG-PEI based Cas9/sgRNA delivery system. Reprinted with permission from ref. 102. Copyright 2017 John Wiley & Sons. Inc.