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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Biomaterials. 2018 Apr 18;171:207–218. doi: 10.1016/j.biomaterials.2018.04.031

Table 1.

Characteristics of different CRISPR/Cas9 genome editing systems.

CRISPR/Cas9 platforms Advantages Disadvantages Non-viral vectors Refs
Cas9 protein and sgRNA swift onset transient duration low off-target effects high cost endotoxin-contamination Lipids CPP polymers DNA nanoclew Au nanoparticles zeolitic imidazole frameworks 69,96–99,102,103,110,113,119
Cas9 mRNA and sgRNA quick onset transient expression low off-target effects poor stability lipids zwitterionic amino-lipid nanoparticles 104,105,112,116
CRISPR/Cas9 plasmid low cost great stability integration risk low efficiency delayed onset lipids CPP polymers 100,101,117,118