FIGURE 4.

Growth profiles and lycopene production in response to overnight and inoculation induction of the mevalonate pathway. (A) When the mevalonate pathway is induced during overnight growth (O/N, dashed lines), mevalonate-expressing strains suffer from growth inhibition. The growth inhibition subsides at the end of the time course for the mevalonate-only strain, but persists for the strain also expressing lycopene. All strains have little to no growth inhibition when mevalonate is induced at inoculation (IND, solid lines). (B) OD-normalized lycopene production is greater for the mevalonate-expressing strains regardless of induction time, but overnight induction yields lower OD-normalized lycopene production than inoculation induction. Error bars represent standard deviation.