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. 2018 May 3;9:760. doi: 10.3389/fmicb.2018.00760

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Copyright © 2018 Miguez, McNerney and Styczynski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Principal component analysis of overnight- and inoculation-induced pLac32EBI + pBadMEV, pLac32Ø + pBadMEV, and pLac32EBI + pBadØ metabolomics data. (A) In pLac32EBI + pBadMEV strains, there is visible separation between induction conditions across the time course, with induction effects far outweighing temporal effects. (B) Separation is also seen for pLac32Ø + pBadMEV strains between induction conditions, though the magnitude of separation is similar to the magnitude of the temporal effects and there is less distinct separation at hour 6. (C) In pLac32EBI + pBadØ strains, there is little separation at each time point, indicating no metabolic distinction between induction conditions. “O/N” indicates overnight induction; “IND” indicates inoculation induction. All colored ellipses represent 95% confidence intervals for each group.