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. 2018 May 3;9:760. doi: 10.3389/fmicb.2018.00760

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Copyright © 2018 Miguez, McNerney and Styczynski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Levels of metabolites of interest plotted over time. (A) Homocysteine levels for overnight- and inoculation-induced pLac32EBI + pBadMEV strains have different trends based on induction condition. The overnight-induced strain appears to maintain higher homocysteine levels over the time course than wild type does. (B) Trends similar to this occur in homocysteine levels for pLac32Ø + pBadMEV strains at each induction condition, except in the overnight-induced strain at hour 6. (C) Homocysteine levels are almost identical at all time points for both overnight- and inoculation-induced pLac32EBI + pBadØ strains. (D) Homoserine levels in pLac32EBI + pBadMEV strains have similar trends to the ones seen in homocysteine. (E) Cysteine sulfinic acid and (F) ethanolamine trends are nearly identical to those in homoserine for pLac32EBI + pBadMEV strains. “O/N” indicates overnight induction; “IND” indicates inoculation induction. Box and whisker plots depict the normalized peak areas. Black lines are the medians, and boxes are the middle 50% values. Error bars represent standard deviation.