Fig. 2. CD spectroscopy of coiled-coil titration experiments. (A) Titration of N-B3.5 to N-A4B3, (B) titration of N-B3 to N-A4B3.5, (C) titration of N-A3.5 to N-A3B4, (D) titration of N-A3 to N-A3.5B4, (E) titration of C-B3.5 to C-A4B3, (F) titration of C-B3 to C-A4B3.5, (G) titration of C-A3.5 to C-A3B4, (H) titration of C-A3 to C-A3.5B4, (0 eq. Pcomp – blue, 0.5 eq. Pcomp – magenta, 1 eq. Pcomp – red, reference spectrum for complete displacement by Pcomp – black, dashed line). Conditions: 50 μM N/C-AxBy, PBS, 20 °C. CD data of the displaced coil peptide was used for baseline correction.
