Table 2. Cytotoxicity assessed in 3D cultures. Spheroids (2.5 103 cells per well) were treated with tested compounds. The growth inhibitory effect was evaluated after 72 hours by means of APH test. IC50 values were calculated from the dose–survival curves by four parameter logistic model (P < 0.05).
| Class | IC50 (μM) |
|
| Compound | PSN-1 3D | |
| COXi, PDKi | CAD | 3.73 ± 0.35 |
| CID | 5.25 ± 0.71 | |
| HDACi, COXi | CPA | 7.75 ± 0.01 |
| CPI | 3.63 ± 0.53 | |
| CVA | 0.79 ± 0.06 | |
| CVI | 0.93 ± 0.46 | |
| HDACi, PDKi | CPD | 0.95 ± 1.12 |
| CVD | 3.38 ± 0.88 | |
| Reference | CDDP | 52.56 ± 3.78 |