Fig. 7. Top-view fluorescent images of cultured cells on sub-μm PDMS through-hole membranes. (a) Live/dead staining combined with nuclei staining (NucBlue™) after 7 days shows a relatively high number (∼94%) of viable cells (green) with a relatively low number of dead cells (red). (b) This merged image of the PDMS membrane (grayscale after bright field imaging) and an ActinGreen™ and NucBlue™ staining, shows the spread morphology of the cells on the membrane. Scale bars represent (a) 400 μm and (b) 50 μm.
