Fig. 1.

The effect of AAP10 on the number of multinuclear cells. Multinuclear TRACP positive cells (arrow heads) formed in both BM and PB cultures (a). The cells were grown on human bone slices and stained with Hoechst 33258 (blue) and an acid phosphatase leukocyte kit to visualize TRACP (red). Acidosis increased multinuclear cell number in BM cultures, but did not have an effect in PB cultures (b). In BM cultures AAP10 increased multinuclear cell number at physiological pH 7.4, but no effect was seen when compared to control in acidic pH 6.5. The effect was similar in PB cultures at physiological pH 7.4, as AAP10 increased the multinuclear cell number, but in acidosis, AAP10 decreased the number of multinuclear cells. The data are shown as mean ± SEM. AAP10: 100 μM AAP10 in the cultures. **p < 0.01, ***p < 0.001. Scale bar 50 μm.