Skip to main content
. 2018 Apr 25;11:538–553. doi: 10.1016/j.omtn.2018.04.007

Figure 2.

Figure 2

The Existence of Regulations in the ASH1-miR-375-YWHAZ Signaling Axis

(A) SYBR Green qRT-PCR analysis of ASH1 mRNA after transfected with siRNA or pcDNA3.1 vectors in SMMC-7721 cells. (B) The expression of miR-375 or YWHAZ after knockdown or overexpression of ASH1 in SMMC-7721 cells is shown. (C) Predicted consequential pairing of target region of YWHAZ and miR-375 is shown. (D) The expression of YWHAZ protein was detected by western blot in 3 hepatoma cell lines after the transfection of miR-375. Quantitative analysis of the western blot was performed by ImageJ software, and relative densitometric quantification of YWHAZ proteins was shown. (E) Relative expression of YWHAZ detected by SYBR Green qRT-PCR in three hepatoma cell lines after 48 hr transfected with miR-375 mimics or miR-NC is shown. (F) Luciferase assay in HeLa cell is shown. miR-375 mimics were co-transfected with pLUC-YWHAZ vector or pLUC-mutYWHAZ vector. Relative repression of luciferase expression was standardized to β-gal signal. *p < 0.05.