Figure 5.

miR-375 and YWHAZ Showed Opposite Effects on HCC Cells

(A) TaqMan qRT-PCR was used to evaluate the relative expression of miR-375 in three hepatoma cell lines transfected with miR-375 mimics or miR-NC. The average miRNA expression in miR-NC group was designated as 1. U6 was used as an internal control. (B) Cell proliferation was detected at 24 hr, 48 hr, and 72 hr by Cell Counting Kit-8 assay. (C) (a) shows the representative results of colony formation using the indicated clones. (b) histogram indicates the number of colony formation using the indicated clones. (D) (a) and (c) represent the results of cell invasion and migration across an 8-μm-pore-size membrane with or without Matrigel. (b) and (d) histograms indicate the number of cell invasions and migrations. *p < 0.05.