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. 2018 Mar 7;8(1):1–11. doi: 10.1556/1886.2018.00002

Supplementary material 2.

Isolated Enterobacteriaceae with resistance against 3rd generation methoxyimino cephalosporins or carbapenems, and nonfermentative Gram-negative rod-shaped bacteria, from the environment of Libyan patients at the Bundeswehr Hospital of Hamburg.

Isolates from the environment of Libyan patients at the Bundeswehr Hospital of Hamburg
Enterobacteriaceae with resistance against 3rd generation methoxyimino cephalosporins or carbapenems
Ward Room number Sample I.D. Species Isolation site Day of isolation In rep-PCR 95% identical with In NGS-based MLSTa/ANIb identical with NGS-based MLST type
HBG-2 Room 4 20 K. pneumoniae hands Day 11 18 (HBG-L4), 26 (room 4 HBG-2) 8 (HBG-L2)a,b, 18 (HBG-L4)a,b, 26 (room 4 HBG-2)(a),(b) 18 (HBG-L4), 26 (room 4 HBG-2)(a),(b)
HBG-2 Room 3 21 K. pneumoniae hands Day 11 None
HBG-2 Room 4 26 K. pneumoniae handle of a bag Day 11 18 (HBG-L4), 20 (room 4 HBG-2) 8 (HBG-L2)(a),b, 18 (HBG-L4)(a),b, 20 (room 4 HBG-2)(a),b 18 (HBG-L4), 20 (room 4 HBG-2)
HBG-2 Room 1 27 K. pneumoniae hands and beard Day 11 16 (HBG-L3), 28 (room 3 HBG-2) 16 (HBG-L3)a,b, 28 (room 3 HBG-2)a,b 16 (HBG-L3), 28 (room 3 HBG-2)
HBG-2 Room 3 28 K. pneumoniae walking frame Day 11 16 (HBG-L3), 27 (room 1 HBG-2), 16 (HBG-L3)a,b, 27 (room 1 HBG-2)a,b 16 (HBG-L3), 27 (room 1 HBG-2),
Nonfermentative rod-shaped bacteria
Ward Room number Sample I.D. Species Isolation site Day of isolation In rep-PCR 95% identical with In NGS-based MLSTa/ANIb identical with NGS-based MLST type
HBG-2 Room 3 19 P. aeruginosa walker Day 11 29 (room 3 HBG-2)b
HBG-2 Room 3 29 P. aeruginosa hands Day 11 19 (room 3 HBG-2)b
HBG-1-ITS Room 1 30 P. putida sink (spout) Day 11 None

a Completely identical based on MLST

(a) identical based on MLST but not all markers found/complete due to lack of coverage.

b Identical based on ANI (>= 99.9%)

(b) highly similar based ANI (>= 99.5%, < 99.9%).