Fig. 5. Intracellular fluorescence change caused by drug treatment. (A) HeLa cells were incubated with 500 μM H2O2 with or without 2 mM NAC for 6 h and stained with 20 μM TCFCl-GSH for 20 min. Quantitative data of fluorescence intensity was calculated by FV10-ASW 4.0 software and measured per one cell. Results are expressed as mean ± standard deviation of three independent experiments. Ex. 559 nm/em. 575–675 nm. Scale bar: 20 μm.
