Figure 1.

The B-dependent decrease in the MSL of growing B-deficient C. album cells. Boric acid (100 μm) was added to a growing suspension of B-deficient C. album cells 2 d after their subcultivation. The cells were collected at the times shown and then denatured with ethanol. The denatured and rehydrated cells (from 1 g fresh weight) were equilibrated for 30 min with a polydisperse dextran probing solution (1 mL). The molecular size distribution of the dextrans was modified by their partial diffusion into the cell lumina. The modified dextran solution was fractionated by SEC and the eluate monitored with a polarimetric detector. The dextran partition curves were generated by computer analysis of the size-exclusion chromatograms (Dautzenberg et al., 1999). The curves show the dependence of the dextran partition coefficient on Stokes' radius of the dextran. The Stokes' radius obtained from each curve at a partition coefficient of 0.5 is designated as the MSL of the walls.