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. 1999 Nov;121(3):829–838. doi: 10.1104/pp.121.3.829

Figure 3.

Figure 3

SEC with refractive index (RI) detection and SEC with ICP-MS detection of the saponified and polygalacturonase-treated phosphate buffer-soluble extracts of the AIR from C. album cells grown in the presence or absence of boric acid. A, SEC-RI profile of the extract from cells grown in the presence of boric acid (100 μm). B, SEC-RI profile of the extract from cells grown in the absence of boric acid. C, SEC-RI profile of the extract from growing B-deficient cells that had been treated for 10 min with 10B boric acid (100 μm). The RG-II dimer (dRG-II-B) and monomer (mRG-II) eluted at 21.9 and 23.8 min, respectively. The insets in A through C show the 11B and 10B profiles obtained by SEC-ICP-MS analysis of the extracts. The ICP-MS was operated in the selected ion mode to detect only the 11B and 10B isotopes. The peak at approximately 36 min corresponds to boric acid that originated from a contaminant in the eluant used for chromatography. The Superdex-75 column was calibrated with red wine dRG-II-B (approximately 9.4 kD) and red wine mRG-II (approximately 4.7 kD), which have retention times of 22.8 and 24.7 min, respectively. Dextrans of 40 and 25 kD eluted at 17 and 19.6 min, respectively. The Vi of the column using Glc was 35 min.