. 1999 Nov;121(3):829–838. doi: 10.1104/pp.121.3.829

Table I.

Glycosyl residue composition and RG-II and B contents of the AIR isolated from C. album cells grown in the presence or absence of boric acid

Glycosyl Residue	B Normal^a	B-Deficient^b	¹⁰B-Treated^c
		mol %^b
Rha	9	8	9
Fuc	2	2	2
Ara	18	17	19
Xyl	7	11	11
Man	1	1	1
Gal	13	11	10
Glc	27	30	30
GalA	23	19	19
		μm g⁻¹ dry wt AIR
mRG-II^d	0.6	4.3	2.3
dRG-II-B^d	1.8	0	1.0
B^e	1.6	<0.1	1.0

The values given are for those glycosyl residue that accounted for >1 mol % of the AIR. The glycosyl residues that are diagnostic of RG-II (2-O-Me Fuc, 2-O-Me Xyl, and apiose) each accounted for <0.5 mol % of the AIR.

B, AIR from cells grown in the presence of ¹¹B boric acid (100 μm).

AIR from cells grown in the absence of added boric acid.

AIR from B-deficient cells grown for 10 min in the presence of ¹⁰B boric acid (100 μm).

The RG-II content was estimated from the 2-O-Me Fuc and 2-O-Me Xyl contents of the AIR. The relative proportions of dRG-II-B and mRG-II were determined by SEC of the phosphate-buffer-soluble extracts of the AIR (see Fig. 3), and these values were used to estimate the mRG-II and dRG-II-B contents using molecular masses of 5 and 10 kD, respectively (O'Neill et al., 1996).