Table II.
Glycosyl residue compositions of the mRG-II and dRG-B-II isolated from C. album cells grown in the presence or absence of boric acid
| Glycosyl Residue | B Normala
|
B-Deficientb
|
10B-Treatedc
|
||
|---|---|---|---|---|---|
| dRG-II-Bd | mRG-IId | mRG-IId | dRG-II-Bd | mRG-IId | |
| mol % | |||||
| 2-MeFuc | 8 | 7 | 7 | 8 | 9 |
| Rha | 15 | 14 | 13 | 13 | 11 |
| Fuc | 4 | 3 | 2 | 4 | 3 |
| 2-MeXyl | 8 | 7 | 8 | 9 | 8 |
| Ara | 24 | 26 | 29 | 24 | 30 |
| Api | 9 | 8 | 11 | 9 | 8 |
| AceA | 7 | 5 | 6 | 6 | 6 |
| Gal | 25 | 30 | 24 | 27 | 25 |
| 11B/10Be | 3.8 | –f | –f | 0.6 | –f |
RG-II was isolated by SEC from the phosphate buffer-soluble extracts of the AIR of C. album cells grown in the presence or absence of boric acid (100 μm). The neutral glycosyl residue compositions were determined by gas-liquid chromatography analysis of the alditol acetates.
Cells grown continuously in the presence of 11B boric acid (100 μm).
Cells grown continuously in the absence of added boric acid.
B-deficient cells that were grown for 10 min in the presence of 10B boric acid (100 μm).
mRG-II and dRG-II-B were isolated from the phosphate-buffer-soluble fractions by SEC (see Fig. 3).
The relative abundance of 11B and 10B in dRG-II-B were determined by SEC-ICP-MS analysis of the phosphate-buffer-soluble fractions.
mRG-II contains no detectable amounts of B.