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. 1999 Nov;121(3):829–838. doi: 10.1104/pp.121.3.829

Table IV.

The effect of EDTA in the presence or absence of boric acid on the wall pore size of C. album cells

Time/Treatment MSL
nm
2.5 h
 pH 5.5 3.6
 EDTA 4.3
 EDTA + 1 mm B 3.9
 EDTA + 10 mm B 3.8
20 h
 pH 5.5 3.8
3.9
 EDTA 6.4
7.1
 EDTA + 1 mm B 5.4
4.7
 EDTA + 10 mm B 4.2
NDa

C. album cells were grown in the presence of boric acid (100 μm) and then extracted with ethanol. The denatured cells (5 g) were rehydrated in water and then incubated for the indicated times in 20 mm Na phosphate, pH 5.5. (45 mL), containing 50 mm EDTA in the presence or absence of boric acid. The cells were then transferred to 20 mm Na phosphate, pH 6.5, and the MSL of their walls determined.

a

ND, Not determined.