Table V.

The effect of pH, divalent cations, and divalent cation chelators in the presence or absence of boric acid on the amount of mRG-II formed from dRG-II-B

Treatment	mRG Formed
	% of total RG-II
None	2
pH 2.0	100
pH 2.0 + 1 mm boric acid	95
pH 2.0 + 10 mm boric acid	68
pH 2.0 + 1 mm boric acid + 0.5 mm Pb2+	42
pH 2.0 + 10 mm boric acid + 0.5 mm Pb2+	11
pH 2.0 + 1 mm boric acid + 0.5 mm Ca2+	95
pH 2.0 + 10 mm boric acid + 0.5 mm Ca2+	62
50 mm EDTA pH 5.5	13
50 mm EDTA pH 5.5 + 1 mm boric acid	9
50 mm EDTA pH 5.5 + 10 mm boric acid	8
25 mm CDTA pH 5.5	33
25 mm CDTA pH 5.5 + 1 mm boric acid	28
25 mm CDTA pH 5.5 + 10 mm boric acid	18

Solutions (200 μL) containing dRG-II-B (500 μg) were treated for 24 h at the specified conditions and the amounts of mRG-II that had formed then determined by SEC.