Figure 5.

NOX2 regulates hepatic immune cell inflammatory vigor. Eight‐week‐old WT and NOX2^−/− mice were fed an HFD for 22 weeks, and cytokine production in liver infiltrating immune cells was quantified by flow cytometry. Cells were exclusively identified from neutrophil, macrophage, and CD3⁺CD4⁺ gates described in Fig. 4G. (A) Cytokine production by liver‐infiltrating neutrophils (CD11b⁺GR1^high). (B) Cytokine production by liver‐infiltrating macrophages (CD11b⁺GR1^low). (C) Cytokine production by liver‐infiltrating T cells (CD3⁺CD4⁺). White bars denote WT mice; black bars denote NOX2^−/− mice. (A‐C) A representative of two individual experiments, n = 4‐6 mice/condition. Data represent means + SE. Student t test, *P < 0.05, **P < 0.01.