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. Author manuscript; available in PMC: 2019 May 3.
Published in final edited form as: Cell Stem Cell. 2018 May 3;22(5):726–739.e7. doi: 10.1016/j.stem.2018.04.005

Figure 4. PRC1 is required for the expression of epidermal and hair follicle developmental genes.

Figure 4

(A) Overlap of significantly downregulated genes between I53A and 2KO epidermis. (B) GO analysis of downregulated genes in 2KO epidermis. Selected GO terms are in blue. (C) RT-qPCR analysis of critical epidermal genes in FACS-enriched 2KO and I53A epidermal cells. n=3. (D) IGV browser views of ChIP-seq of Ring1b, H2AK119ub, H3K27me3, H3K27ac, H3K4me3, Rybp, and input for indicated genes in control epidermal progenitors. (E–F) ChIP-qPCR showing the binding of Ring1b (E) and H3K27ac (F) in control epidermal progenitors. Data are mean ±SEM, n=2. (G) Overlap of significantly downregulated genes between I53A and 2KO hair follicle cells. (H) RT-qPCR analysis of hair follicle lineage genes in 2KO and I53A HFs. n=3. (I–L) ChIP-qPCR showing the binding of Ring1b and H3K27ac to hair follicle lineage genes in hair follicle stem cells (HF-SC; I-J), outer root sheath cells (ORS; K), and matrix/transient amplifying cells (Mx; L). Data are mean ±SEM, n=2. Data in graphs (C and H) are mean ±SEM. *p<0.05; **p<0.01; ***p<0.001 (two-sided t test). See also Figure S5 and Tables S2 and S3.