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. Author manuscript; available in PMC: 2019 May 3.
Published in final edited form as: Cell Stem Cell. 2018 May 3;22(5):726–739.e7. doi: 10.1016/j.stem.2018.04.005

Figure 5. Ring1b binding positively regulates gene expression.

Figure 5

(A–C) PRC1 loss-of-function analyses in K14-CreER; Ring1a−/− Ring1bflox/flox epidermal progenitor culture upon 4-OHT treatment. (A) Western blot showing changes in Ring1b and H2AK119ub protein levels over time. (B) ChIP-qPCR showing changes in Ring1b binding. Data are mean ±SEM, n=3. (C) RT-qPCR analysis of changes in the expression of Ring1b target genes. Data are mean ±SEM, n=3. (D–F) Ring1b overexpression analyses in wild type epidermal progenitor culture. (D) RT-qPCR analysis of endogenous and total Ring1b mRNA. (E) ChIP-qPCR showing changes in Ring1b binding. n=3. (F) RT-qPCR analysis of changes in expression of Ring1b target genes. n=5. Data in graphs (E and F) are mean ±SEM. *p<0.05; **p<0.01; ***p<0.001 (two-sided t test). See also Figure S6.