Key Resources Table.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| α-HA.11 Clone:16B12 | BioLegend | Cat# 901514; RRID:AB_2565336 |
| α-Pgk1 (Phosphoglycerate Kinase monocolonal) | Novex (Life Technologies) | Part# 459250; RRID:AB_2532235 |
| α-v5 monoclonal | Invitrogen | Cat# 46-0705; RRID:AB_2556564 |
| α-mouse HRP-conjugated secondary | GE Healthcare | Cat# NA931-1ML |
| α-V5-coupled agarose | Sigma Aldrich | Cat# A7345-1ml |
| Rat α-tubulin alpha | Bio-Rad | Cat# MCA77G |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Acid-washed glass beads, 425–600μm | Sigma Aldrich | G8772-500G |
| Imperial Blue Protein Stain | Thermo Fisher | Cat# 24615 |
| Halt Protease Inhibitor | Thermo Fisher | Cat# 1861279 |
| 0.5mm dia Zirconia/silica beads | BioSpec Products | Cat# 11079105z |
| Hybond H+ membrane | GE Healthcare | Prod# RPN203B |
| Illustra Probequant Columns | GC Healthcare | Prod#28903408 |
| ProlongGold w/DAPI | LifeTechnologies | Cat#P36935 |
| Critical Commercial Assays | ||
| Amersham MegaPrime DNA Labeling Kit | GE Healthcare | Prod#RPN1604 |
| Experimental Models: Organisms/Strains | ||
| S. cerevisiae: Strain background: SK1 (ho::LYS2, lys2, ura3, leu2::hisG, his3::hisG, trp1::hisG) | ATCC (Kane and Roth, 1974) | ATCC: 204722 |
|
A15055: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX |
Berchowitz/Amon labs | N/A |
|
A33024: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-3V5::HIS3MX6/RIM4-3V5::HIS3MX6 IME2st/IME2st |
Berchowitz/Amon labs | N/A |
|
A37489: MATa/α RIM4-EGFP::HIS3/RIM4-EGFP::HIS3 HTB1-mCherry-HISMX6/+ |
Berchowitz/Amon labs | N/A |
|
A38060: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-99A-3V5::HIS3MX6/RIM4-99A-3V5::HIS3MX6 |
This paper | N/A |
|
A38072: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-10A-3V5::HIS3MX6/RIM4-10A-3V5::HIS3MX6 |
This paper | N/A |
|
A38075: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-47A-3V5::HIS3MX6/RIM4-47A-3V5::HIS3MX6 |
This paper | N/A |
|
A38932: MATa/α rim4-47A-EGFP::HIS3/rim4-47A-EGFP::HIS3 HTB1-mCherry-HISMX6/HTB1-mCherry-HISMX6 |
This paper | N/A |
|
B5: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-47A-3V5::HIS3MX6/RIM4-47A-3V5::HIS3MX6 IME2st/IME2st |
This paper | N/A |
|
B48: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-3V5::HIS3MX6/RIM4-3V5::HIS3MX6 |
Berchowitz/Amon labs | N/A |
|
B67: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-52A-3V5::HIS3MX6/RIM4-52A-3V5::HIS3MX6 |
This paper | N/A |
|
B70: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-36A-3V5::HIS3MX6/RIM4-36A-3V5::HIS3MX6 |
This paper | N/A |
|
B73: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-16A-3V5::HIS3MX6/RIM4-16A-3V5::HIS3MX6 |
This paper | N/A |
|
B119: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-3V5::HIS3MX6/+ |
This paper | N/A |
|
B120: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-47A-3V5::HIS3MX6/+ |
This paper | N/A |
|
B123: MATa/α ura3::pTUB1-GFP-TUB1::URA3/ura3::pTUB1-GFP-TUB1::URA3 SPC42-mCherry::NATMX6/SPC42-mCherry::NATMX6 ZIP1-GFP(700)/ZIP1-GFP(700) RIM4-3V5::HIS3MX6/RIM4-3V5::HIS3MX6 |
This paper | N/A |
|
B126: MATa/α ura3::pTUB1-GFP-TUB1::URA3/ura3::pTUB1-GFP-TUB1::URA3 SPC42-mCherry::NATMX6/SPC42-mCherry::NATMX6 ZIP1-GFP(700)/ZIP1-GFP(700) RIM4-47A-3V5::HIS3MX6/RIM4-47A-3V5::HIS3MX6 |
This paper | N/A |
|
B129: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-21A-3V5::HIS3MX6/RIM4-21A-3V5::HIS3MX6 |
This paper | N/A |
|
B149: MATa/α ura3::pTUB1-GFP-TUB1::URA3/ura3::pTUB1-GFP-TUB1::URA3 SPC42-mCherry::NATMX6/SPC42-mCherry::NATMX6 ZIP1-GFP(700)/ZIP1-GFP(700) RIM4-47A-3V5::HIS3MX6/RIM4-3V5::HIS3MX6 |
This paper | N/A |
|
B152: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-27A-3V5::HIS3MX6/RIM4-27A-3V5::HIS3MX6 |
This paper | N/A |
|
B155: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-25A-3V5::HIS3MX6/RIM4-25A-3V5::HIS3MX6 |
This paper | N/A |
|
B197: MATa/α ura3::pTUB1-GFP-TUB1::URA3/ura3::pTUB1-GFP-TUB1::URA3 SPC42-mCherry::NATMX6/SPC42-mCherry::NATMX6 RIM4-3V5::HIS3MX6/RIM4-3V5::HIS3MX6 |
This paper | N/A |
|
B198: MATa/α ura3::pTUB1-GFP-TUB1::URA3/ura3::pTUB1-GFP-TUB1::URA3 SPC42-mCherry::NATMX6/SPC42-mCherry::NATMX6 RIM4-47A-3V5::HIS3MX6/RIM4-47A-3V5::HIS3MX6 |
This paper | N/A |
|
B207: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-3V5::HIS3MX6/RIM4-3V5::HIS3MX6 RPN6::rpn6-1::HIS3MX6/RPN6::rpn6-1::HIS3MX6 |
This paper | N/A |
|
B251: MATa/α ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1/pGAL-NDT80::TRP1 CLB3-3HA::KANMX6/CLB3-3HA::KANMX RIM4-3V5::HIS3MX6/RIM4-3V5::HIS3MX6 RPN6::rpn6-1::HIS3MX6/RPN6::rpn6-1::HIS3MX6 IME2st/IME2st |
This paper | N/A |
|
B343: MATa/α ura3::pGPD1-GAL4(848).ER::URA3 ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1, pGAL-NDT80::TRP1 CLB3-3HA:KANR CLB3-3HA:KANR rim4Δ::HIS3MX6 rim4Δ::HIS3MX6 |
This paper | N/A |
|
B346: MATa/α ura3::pGPD1-GAL4(848).ER::URA3 ura3::pGPD1-GAL4(848).ER::URA3 pGAL-NDT80::TRP1, pGAL-NDT80::TRP1 CLB3-3HA:KANR CLB3-3HA:KANR rim4-45E-3V5::HIS3MX6 rim4-45E-3V5::HIS3MX6 |
This paper | N/A |
| Oligonucleotides | ||
| Primer: CLB3 Northern blot probe forward: AGC ATA AGT AAG CCA AAA GTC GC | (Carlile and Amon, 2008) | N/A |
| Primer: CLB3 Northern blot probe reverse: C TAA TGC TAT CCA CTT CGC TAC G | (Carlile and Amon, 2008) | N/A |
| Recombinant DNA | ||
| RIM4-allA-HIS3 plasmid (pLB90) | This paper | N/A |
| RIM4-allE-HIS3 plasmid (pLB119) | This paper | N/A |
| Software and Algorithms | ||
| GraphPad Prism | GraphPad software | https://www.graphpad.com/scientific-software/prism/ |
| Proteome Discoverer | Thermo Fisher | OPTON-30795 |
| Mascot version 2.4.1 | Matrix Science | http://www.matrixscience.com/ |
| Other | ||
| CellAsic ONIX microfluidic system | Millipore Sigma | Model EV-261 |
| CellAsic ONIX microfluidic diploid yeast plates | Millipore Sigma | Cat #: Y04D-02-5PK |
| DeltaVision Elite Microcope | GE Healthcare | Prod #: 29065728 |