Table I.

Total Rubisco activity following recovery assays

Extract	Time	Total Activity	Percentage of 3 pm Control
		μmol CO2 m−2 s−1
Control	9 am	20.9  ± 0.23	75
	3 pm	27.2  ± 0.25	100
200 mm Malate	3 pm	27.8  ± 0.28	102
Control mix	9 am + 3 pm	23.5  ± 0.29	86

Rubisco total activities were measured for morning (9 am) and afternoon (3 pm) control extracts, or after spiking afternoon samples with 200 mm malate or in a mixture comprising 50% morning and 50% afternoon extract. The data are expressed as the means ± se of total Rubisco activity following an 8-min incubation (n = 5) and as mean percentages of the maximum total activity. Desalting morning and afternoon extracts had no discernible effect on either initial or total activities (data not shown).