Table 1.
Distribution of PRV-152-labeled neurons following injection into the rectum wall
| Areas | PRV-152-positive neurons | ||||
|---|---|---|---|---|---|
|
| |||||
| d3 | d4 | d5 | d6 | d7 | |
| Spinal cord | +/- | + | + | + | |
| RPa | +/- | + | + | ++ | + |
| RVMM (RMg+LPGi) | +/- | + | ++ | +++ | ++ |
| RVL | + | ++ | +++ | + | |
| A5 | + | + | + | + | |
| Sol | + | + | ++ | +/- | |
| DMV | +/- | + | + | +/- | |
| Amb | +/- | + | + | +/- | |
| LC | + | ++ | +++ | + | |
| SubCD | + | ++ | +++ | + | |
| Gi | +/- | ++ | ++ | +/- | |
| Icp | +/- | + | + | +/- | |
| LPN | + | +++ | +++ | + | |
| PRN | ++ | ++ | ++ | +/- | |
| PAG, ventrolateral | ++ | ++ | +++ | + | |
| PAG, dorsal | + | ++ | ++ | + | |
The number of PRV-152-positive neurons was qualitatively estimated in the whole brains of mice. Semi-quantitative estimates of the signals are indicated as follows: +++ (high: more than 20 PRV-152-positive neurons per brain section); ++ (moderate: between 10 and 20 PRV-152-positive neurons per brain section); + (low: less than 10 PRV-152-positive neurons per brain section); +/- (low, inconsistent staining across the animals: less than 5 PRV-614-positive neurons per brain section in most animals but with no staining observed in some animals); and - (No PRV-152-positive neurons). Amb: the nucleus ambiguous, LC: locus coeruleus. Gi: the gigantocellular reticular nucleus, LPGi: lateral paragigantocellular, RVL: rostral ventrolateral reticular nucleus, Sol: solitary tract nucleus, RMg: raphe magnus nucleus, SubCD: subcoeruleus nucleus, RPa: raphe pallidus nucleus, Icp: inferior cerebellar peduncle, PAG: periaqueductal gray, LPN: lateral parabrachial nucleus, PRN: Pontine reticular nucleus.