Figure 3. Immunolabelling and Western blot analysis of FKRP_MD muscle.

Transverse 10µm cryosections from 12 week old wildtype (A,C,E) and FKRP_MD (B,D,F) mouse triceps were labelled with Hoescht 33342 to visualise the nuclei (A-B) and an antibody against laminin α2 (C-D), with a colour composite shown in E-F. Laminin α2 immunolabelling was variable in the FKRP_MD with small clusters of fibres showing an increase relative controls whilst the majority of other fibres displayed either similar levels to controls or a slight decrease. Scale bar represents 50µm. Western Blotting analysis (G) of quadriceps from wildtype and FKRP_MD mice shows IIH6 labelling, present in WT mice (lanes 3,4 and 5) was absent in FKRP_MD mice (lanes 1,2,6 and 7). Each lane contains an extract from individual animals. β-DG (43kDa) which also acts as a loading control is shown to be unchanged in the FKRP_MD. The images shown in C and D) are shown using a look up table from Image J to emphasise the variation in intensity of laminin α2 immunolabelling across the section. J shows the scale used.