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. Author manuscript; available in PMC: 2018 May 10.
Published in final edited form as: Hum Mol Genet. 2013 Nov 13;23(7):1842–1855. doi: 10.1093/hmg/ddt577

Figure 5. FKRPMD LARGE histology.

Figure 5

10µm cryosections from FKRPMD (A,C,E,G) and FKRPMDLARGE (B,D,F,H) diaphragm (A-B), soleus (C-D) gastrocnemius (E-F), and tibialis anterior (G-H) at 30 weeks of age stained with haematoxylin and eosin (A-H). All muscles showed a marked variation in fibre size, the presence of degenerative fibres infiltrated with macrophages and an increase in centrally nucleated fibres relative to the FKRPMD. I-K show Alizarin Red labelling of wildtype (I), FKRPMD (J) and FKRPMDLARGE (K) diaphragm, with a greater incidence of calcium deposits (red) observed in the FKRPMDLARGE relative to FKRPMD. (L) is an image of a 30 week old FKRPMDLARGE gastrocnemius, showing an example of split muscle fibres. In images A-H and L scale bar represents 50µm. In I-K scale bar represents 100µm.