Fig 2. The uptake capacity of GM-CSF driven myeloid cells increased as cell progress in development and modestly decreased in more differentiated cell types.

Sorted cell populations were fed 0.5 mg/mL Dextran Alexa Flour 488 (10,000 MW) or S. aureus BioParticle Alexa flour 594 conjugate for 1 h at 37°C (red), or at 4°C (blue) as negative control. The uptake of the fluorescent tracers was measured by flow cytometry. (A) Representative histograms of Dextran uptake (top panels) and BioParticle uptake (bottom panels) by the five isolated myeloid cell populations. Uptake of dextran (B) and bioparticles (C). Uptake values were calculated by subtracting the MFI of the ice control for each population from the MFI of that population incubated at 37°C. These data are compiled from at least four independent experiments. Statistical analysis was performed using SAS, one-way ANOVA Tukey’s multiple comparison tests. Letters over bars indicate statistically significant differences in means (p<0.05).